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VHE2.2 [VHE 2.2]
VHE2.2 [VHE 2.2]
規(guī)格:
貨期:
編號:B215739
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 VHE2.2 [VHE 2.2]
商品貨號 B215739
Designations VHE2.2 [VHE 2.2]
Species Homo sapiens, human
Vector
Construct size (kb): 6.800000190734863
Insert
DNA: genomic
Insert lengths(kb): 2.200000047683716
Gene product: immunoglobulin, heavy, variable, VH3 class [IGHV3*]
Alleles: D2, D1
Insert Size (kb) 2.200
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--4.4, 2.25; PstI--4.75, 1.9; HincII--3.5 (doublet); BamHI--4.75, 2.0; HindII--6.8.
This probe identifies a VH3 gene subclass. This probe flanks p00803.
The approximate number of the VH3 class of genes is 24.
Detects polymorphisms with EcoRI, SacI, ApaI, HindIII, AvaII, RsaI, TaqI, and BglII. The greatest variation is seen with EcoRI.
There are three polymorphic loci detected with EcoRI: (1) 6.7 kb/no band; (2) 6.0 kb/4.6 kb; and (3) 3.5 kb/no band.
VHE2.2 is a subclone of lambda VH26.
References

Matthyssens G, Rabbitts TH. Structure and multiplicity of genes for the human immunoglobulin heavy chain variable region. Proc. Natl. Acad. Sci. USA 77: 6561-6565, 1980. PubMed: 6450418

Walter MA, Cox DW. Analysis of genetic variation reveals human immunoglobulin VH-region gene organization. Am. J. Hum. Genet. 42: 446-451, 1988. PubMed: 2894757

Walter MA, et al. Apa I polymorphism of a human immunoglobin VH3 subclass locus. Nucleic Acids Res. 15: 4697, 1987. PubMed: 2884630

Diane W Cox, personal communication

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