產(chǎn)品名稱 |
Orientia chuto |
商品貨號(hào) |
B216732 |
Classification |
Rickettsiaceae |
Agent |
Orientia chuto |
Strain |
Dubai |
Biosafety Level |
3
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Product Format |
frozen 1 mL per vial |
Storage Conditions |
Vapor phase of liquid nitrogen |
Images |
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Comments |
New Taxon. It is recommended to determine viability by a PCR-based assay as agent does not produce cytopathic effects. |
Effect on Host |
Toxicity observed |
Recommended Host |
Vero cells (ATCC® CCL-81™)
|
Growth Conditions |
Temperature: 35°C
Recommendations for Infection: Plate cells 24-36 hours prior to infection and infect when cultures are 70-90% confluent. Remove medium and inoculate with a small volume of inoculum (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI. Centrifuge at 800 x g at room temperature for 75 minutes - 2 hours. End adsorption by adding growth medium.
Incubation: 15 days |
Name of Depositor |
L Izzard, Australian Rickettsial Reference Laboratory |
Source |
Clinical female patient with acute scrub typhus infection, Dubai, United Arab Emirates, 2006. |
References |
Izzard L, et. al. Isolation of a novel Orientia species (O. chuto sp. nov.) from a patient infected in Dubai. J. Clin. Microbiol. 48(12): 4404-4409, 2010. PubMed: 20926708
|