產(chǎn)品名稱 |
Murine leukemia virus |
商品貨號 |
B216845 |
Classification |
Retroviridae, Gammaretrovirus, Mammalian virus group |
Agent |
Murine leukemia virus |
Strain |
Rauscher (biologically cloned) |
Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Isolation |
Mouse spleen |
Product Format |
frozen |
Storage Conditions |
-70°C or colder |
Comments |
Infects mouse embryo fibroblast cultures of Fv-1(b) or Fv-1(n) strains and SC-1 cells. |
Effect on Host |
Yes, in vitro effects: Cytopathic effects (plaque formation) only in XC-assay and in mouse S+L- cells No CPE in NIH3T3 TC; Plaques only on XC-assay and on mouse S+L- cells |
Recommended Host |
NIH/3T3 cells (ATCC CRL-1658) Host of Choice: NIH 3T3 cells Host Range: Mice and mouse cells mouse; mouse cells |
Growth Conditions |
Temperature: 37.0°C Duration: 1 day in TC; 5-9 days for XC-assay |
Name of Depositor |
JW Hartley |
Source |
Mouse spleen |
References |
Rauscher FJ. A virus-induced disease of mice characterized by erythrocytopoiesis and lymphoid leukemia. J. Natl. Cancer Inst. 29: 515-543, 1962. PubMed: 14490596
Hartley JW, et al. Complement fixation and tissue culture assays for mouse leukemia viruses. Proc. Natl. Acad. Sci. USA 53: 931-938, 1965. PubMed: 4287967
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