Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--22.6, 10.0, 7.6, 6.2, 2.5; EcoRI/HindIII--22.6, 10.0, 5.6, 2.8, 2.5, 2.2, 0.47. Verified to contain the correct insert sequence using the following oligonucleotides (5'-3'): CCTCCGTGCCCATGTGCTTATCCAC and CTCAAGAGGCTTACGTACATGG. The predicted product size is (bp): 147. The observed product size is (bp): 147. |