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pS2
pS2
規(guī)格:
貨期:
編號(hào):B218226
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pS2
商品貨號(hào) B218226
Designations pS2
Species Homo sapiens, human
Vector
Construct size (kb): 5.0
Insert
DNA: cDNA
Insert lengths(kb): 0.5590000152587891
Tissue: breast adenocarcinoma MCF7 cell line
Gene product: trefoil factor 1 (breast cancer, estrogen-inducible sequence expressed in) [BCEI]
Alleles: A1, A1, A2, A2
Insert Size (kb) 0.559
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): PstI--4.4, 0.32, 0.24; BamHI--5.0; EcoRI--5.0; HindIII--5.0.
This includes the complete coding region but lacks 15 bp of the 5' untranslated region. It includes a 3' untranslated region of 195 bp with an ATTAAA sequence 14 bp upstream from poly(A).
Enzyme(s) not detecting polymorphism: HindIII, MspI, PvuII, TaqI, XbaI.
References

Moisan JP, et al. A gene expressed in human mammary tumor cells under estrogen control is located in 21q22.3 and defines an RFLP. Cytogenet. Cell Genet. 40: 701-702, 1985.

Jakowlew SB, et al. Sequence of the pS2 mRNA induced by estrogen in the human breast cancer cell line MCF-7. Nucleic Acids Res. 12: 2861-2878, 1984. PubMed: 6324130

Masiakowski P, et al. Cloning of cDNA sequences of hormone-regulated genes from the MCF-7 human breast cancer cell line. Nucleic Acids Res. 10: 7895-7903, 1982. PubMed: 6897676

Brown AM, et al. Activation of pS2 gene transcription is a primary response to estrogen in the human breast cancer cell line MCF-7. Proc. Natl. Acad. Sci. USA 81: 6344-6348, 1984. PubMed: 6593703

Rio MC, et al. Specific expression of the pS2 gene in subclasses of breast cancers in comparison with expression of the estrogen and progesterone receptors and the oncogene ERBB2. Proc. Natl. Acad. Sci. USA 84: 9243-9247, 1987. PubMed: 3321071

Jeltsch JM, et al. Structure of the human oestrogen-responsive gene pS2. Nucleic Acids Res. 15: 1401-1414, 1987. PubMed: 3822834

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