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Size (kb): 4.7789998054504400

Vector: pLACT7 (phagemid)

Promoters: Promoter T7 (phi10)

Construction: pTZ18U, pET21b

Marker(s):ampR

Construct size (kb): 4.77899980545044

Features: marker(s): ampR
promoter: T7 (phi10)
promoter: lac
replicon: f1
replicon: pMB1
repressor gene: lacI
MCS: NdeI...HindIII
terminator: T7 (phi10)

expression vector

vector permitting RNA synthesis in vitro

vector permitting production of single-stranded DNA

Restriction digests of the clone give the following sizes (kb): BamHI-- 4.8; BglI--2.6, 1.3, 1.0; SacI--4.8.

Vector contains the following restriction sites (approximate kb from nt 1): BamHI--0.25; BglI--0.01, 2.47, 3.53; EcoRI--0.24, 0.30; EcoRV--1.86; HindIII--0.22; PvuI--0.05, 3.78; PvuII--0.08, 0.50, 2.01, 2.10; SacI--0.23; SalI--0.23; SphI--0.88.

High copy number phagemid allowing controlled expression from either the T7 or the lac promoter.

Temperature: 37.0°C