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Construct size (kb): 40.0

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pJB8
Intact vector size: 5.400
Type of vector: cosmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: BamHI EcoRI
Polylinker sites:
Construction: HomerI
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
replicon: pMB1
Cross references:

DNA: genomic

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: LGALS1
Genomic copy number: unique
Gene name: lectin, galactoside-binding, soluble, 1
Contains complete coding sequence?: U
Chromosome: 22; Localization: 22 q12-q13.1
Tissue: leukocyte
Type of DNA: genomic
Insert end: MboI
Insert end: MboI
Insert size (kb): 35.0
Cross references: DNA Seq. Acc.: J05303

Insert lengths(kb): 35.0

Tissue: leukocyte

Gene product: lectin, galactoside-binding, soluble, 1 [LGALS1]

Restriction digests of the clone give the following sizes (kb): PstI--4.2, 4.2, 3.0, 3.0, 3.0, 2.35, 2.05, 1.95, 1.9, 1.7, 1.5, 1.35, 1.25, 1.1, 0.92, 0.9, 0.85, 0.85, 0.8, 0.76, 0.72, 0.48, 0.42, 0.35;

PvuII--4.6, 4.4, 4.1, 3.7, 3.7, 3.2, 2.2, 2.0, 1.9, 1.65, 1.25, 1.25, 1.0, 1.0, 0.75, 0.65, 0.6, 0.46, 0.4, 0.4; EcoRI--12.0, 8.0, 7.0, 4.5, 3.3, 1.65, 0.84; EcoRI/BamHI--8.4, 8.4, 4.5, 4.3, 3.65, 2.25, 1.85, 1.78, 1.7, 1.15, 0.8;

EcoRI/PstI--4.2, 3.3, 3.0, 3.0, 2.35, 2.2, 1.75, 1.55, 1.35, 1.2, 1.15, 1.1, 1.0, 0.95, 0.95, 0.84, 0.75, 0.68 +smaller.

The 4428 nt sequenced region includes the following restriction sites (nt from the 5' end): PstI--531, 642, 1011 (all intron 1), 1850 (intron 2); 3218 (intron 3); EcoRI--4211 (exon 4).

IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.

The sequence record begins at a Sp1 binding site consensus approximately 260 bp 5' to the initiator ATG.

Contains the complete gene (4 exons).

Introns 2 and 3 contain Alu repeats.