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pUR18N
pUR18N
規(guī)格:
貨期:
編號(hào):B226336
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pUR18N
商品貨號(hào) B226336
Designations pUR18N
Depositors NC Barbet, AM Carr
Biosafety Level 1
Vector Information
Size (kb): 5.5000000000000000
Vector: pUR18N (plasmid)
Promoters: Promoter lac
Construction: pUR18 (ATCC 77297)
Marker(s):ampR,ura4+
Construct size (kb): 5.5
Features: insert detection: lacZ'
marker(s): ampR, ura4
promoter: lac
replicon: pMB1, ars1
Applications
expression vector
shuttle vector
vector for other uses
vector permitting construction of fusion proteins
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--5.5; BamHI--5.5; PstI--5.5; HindIII--5.5.
Shuttle vector permitting visual detection of recombinants by beta-galactosidase alpha complementation.
The addition of NotI and SfiI sites to the MCS enables unidirectional exonuclease III deletions and facilitates the rescue in E. coli of plasmids where dimerization has occurred.
The order of the major features in this plasmid is: ars1 - ura4 - SfiI/MCS/EcoRI/lacZ' - pMB1 ori - ampR.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Barbet N, et al. Versatile shuttle vectors and genomic libraries for use with Schizosaccharomyces pombe. Gene 114: 59-66, 1992. PubMed: 1587485

Shipped freeze-dried
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