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Construct size (kb): 5.599999904632568

DNA: cDNA

Insert lengths(kb): 2.700000047683716

Tissue: eye, fetal

Gene product: paired box homeotic gene 6 (aniridia, keratitis)( paired box homeotic protein, aniridia locus, aniridia 2 (without Wilms tumor, genitourinary abnormalities, and mental retardation), aniridia 2 (without Wilms' tumor, genitourinary abnormalities, and mental retardation), paired box homeotic gene aniridia) [AN2]

Restriction digests of the clone give the following sizes (kb): EcoRI--4.4, 1.35; PstI--4.4, 1.35; PvuII--2.7, 2.1, 1.0; BglII--5.8; HindIII--5.8; EcoRI/PvuI--3.0, 1.4, 1.1, 0.2; EcoRI/PvuII--2.6, 1.4, 1.0, 0.55, 0.2.

Restriction digests of the clone give the following sizes (kb): NotI/SalI--3.0, 2.7.

The insert spans two adjacent genomic NotI fragments of 325 kb and 1400 kb. The 5' end is in the 325 kb NotI fragment.

Corresponds to the 3' portion of the reported sequence.

Overlaps ph12 (ATCC 65958, 65959).

One of the EcoRI sites on the insert ends was lost during cloning.