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pBS-hCD40L-6A9
pBS-hCD40L-6A9
規(guī)格:
貨期:
編號(hào):B229904
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pBS-hCD40L-6A9
商品貨號(hào) B229904
Designations pBS-hCD40L-6A9
GenBank Number

L07414

Species Homo sapiens, human
Depositors J Gauchat
Vector
Construct size (kb): 4.059999942779541
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript II SK
Intact vector size: 2.961
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
promoter: lac, T7, T3
replicon: pMB1, f1
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CD40LG
Other gene symbols: CD40L
Gene name: CD40 antigen ligand
Contains complete coding sequence?: Y
Chromosome: X; Localization: X q26
Tissue: CD4-positive T-cells
Type of DNA: cDNA
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 1.1
Cross references: DNA Seq. Acc.: L07414
Insert lengths(kb): 1.100000023841858
Tissue: CD4-positive T-cells
Gene product: CD40 antigen ligand [CD40L]
Insert Size (kb) 1.100
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): BamHI--3.8, 0.2; HindIII--3.3, 0.7; NcoI--4.0; PvuII--2.6, 1.0, 0.6; EcoRI--3.0, 1.2.
The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.93; HindIII--0.58; NcoI--0.78; PvuII--0.52.
Contains the complete coding sequence (nt 40-825 of the sequence record).
References

Gauchat JF, et al. Human CD40-ligand: molecular cloning, cellular distribution and regulation of expression by factors controlling IgE production. FEBS Lett. 315: 259-266, 1993. PubMed: 7678552

Jean-Francois Gauchat, personal communication

梅經(jīng)理 17280875617 1438578920
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