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pMCT112
pMCT112
規(guī)格:
貨期:
編號:B237127
品牌:Mingzhoubio

標準菌株
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DNA
RNA

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產(chǎn)品名稱 pMCT112
商品貨號 B237127
Designations pMCT112
Species Homo sapiens, human
Applications
A 1.9 kb SphI fragment of this clone has been used which is devoid of repetitive sequences.
The AccI RFLP is observed under normal wash and hybridization stringencies.
Vector
Construct size (kb): 5.800000190734863
Insert
DNA: genomic
Insert lengths(kb): 3.599999904632568
Gene product: DNA Segment, single copy [D9S15]
Alleles: A1, A2, B1, B2
Insert Size (kb) 3.600
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments
Restriction digests of the clone give the following sizes (kb): HindIII/EcoRI--2.8, 2.0, 0.96, + smaller bands; HindIII--2.8, 2.0, 0.96; EcoRI--6.0, 0.22.
Codominant segregation demonstrated in 58 3-generation families for the MspI polymorphism.
pMCT112 shows linkage to the Friedreich ataxia locus at a combined total LOD score of 25.09 at a recombination fraction of theta = 0.
A single copy fragment of this clone detects the same 450 kb NotI fragment detected by D9S5.
Detects genomic restriction fragments of the following sizes (complete digest, kb): NotI--450; EagI--100; BssHII--100; SacII--100.
A 1.9 kb SphI fragment of this clone has been used which is devoid of repetitive sequences.
The AccI RFLP is observed under normal wash and hybridization stringencies. Pre-association with excess human DNA is required.
Enzyme(s) not detecting polymorphism: BglII, PstI, PvuII, RsaI, TaqI.
Enzyme(s) not detecting polymorphism: BamHI, BclI, DraI, EcoRI, HincII, HindIII, HinfI, HpaI, KpnI, SalI, SstI, StuI, XbaI.
References

Chamberlain S, et al. Genetic homogeneity at the Friedreich ataxia locus on chromosome 9. Am. J. Hum. Genet. 44: 518-521, 1989. PubMed: 2929596

Wallis J, Nakamura Y. A new AccI polymorphism for pMCT112 [D9S15]. Nucleic Acids Res. 17: 4904, 1989. PubMed: 2568612

Pandolfo M, et al. Friedreich ataxia in Italian families: genetic homogeneity and linkage disequilibrium with the marker loci D9S5 and D9S15. Am. J. Hum. Genet. 47: 228-235, 1990. PubMed: 2378348

Hanauer A, et al. The Friedreich ataxia gene is assigned to chromosoem 9q13-q21 by mapping of tightly linked markers and shows linkage disequilibrium with D9S15. Am. J. Hum. Genet. 46: 133-137, 1990. PubMed: 2294745

Fujita R, et al. Physical mapping of two loci (D9S5 and D9S15) tightly linked to Friedreich ataxia locus (FRDA) and identification of nearby CpG islands by pulse-field electrophoresis. Genomics 10: 915-920, 1991. PubMed: 1916823

Carlson M, et al. Isolation and mapping of a polymorphic DNA sequence pMCT112 on chromosome 9q (D9S15). Nucleic Acids Res. 15: 10614, 1987. PubMed: 2892184

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