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pCE30
pCE30
規(guī)格:
貨期:
編號:B244526
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pCE30
商品貨號 B244526
Designations pCE30
Depositors NE Dixon
Biosafety Level 1
Vector Information
Size (kb): 4.0019989013671880
Vector: pCE30 (plasmid)
Promoters: Promoter lambda PL
Construction: pUC9, pMY17-3
Marker(s):ampR
Construct size (kb): 4.001998901367188
Features: marker(s): ampR
promoter: lambda PL, lambda PR
replicon: pMB1
repressor gene: cI857
Applications
expression vector
vector containing primer sites useful for sequencing
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--4.0; BamHI/EcoRI--4.0; BglI--2.9, 1.1.
The sequence surrounding the cloning sites has been used to design primers for direct sequencing, including the M13 universal primer.
Because cI857 is expressed by the plasmid itself, from its natural promoter PM, the vector may be used in virtually any E. coli strain.
Expression vector containing primer sites useful for sequencing and encoding cI857.
Constructed by inserting a 1349 bp fragment from pMY17-3 containing the cI857 gene and tandem lambda PR and PL promoters into pUC9. The promoters are immediately upstream of the cloning sites.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 30.0°C
References

Elvin CM, et al. Modified bacteriophage lambda promoter vectors for overproduction of proteins in Escherichia coli. Gene 87: 123-126, 1990. PubMed: 2139621

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梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
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