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4.25 plasmid in E. coli
4.25 plasmid in E. coli
規(guī)格:
貨期:
編號:B206639
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 4.25 plasmid in E. coli
商品貨號 B206639
Designations 4.25 plasmid in E. coli
Species Tobacco mosaic virus
Depositors WO Dawson
Vector
Construct size (kb): 4.0
Insert
DNA: cDNA
Insert lengths(kb): 1.32
Gene product: end, 3'
Insert Size (kb) 1.320
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Comments
Insert contains the following sites separated by (bp)(approx): HindIII- 195- PvuII- 780- AccI- 350- PstI.
Restriction digests of the clone give the following sizes (kb): HindIII--4.0; PstI--4.0; HindIII/PstI--2.7, 1.35.
The insert spans nucleotides 5080-6395 of TMV.
Classification Tobamovirus
References

Dawson WO, et al. cDNA cloning of the complete genome of tobacco mosaic virus and production of infectious transcripts. Proc. Natl. Acad. Sci. USA 83: 1832-1836, 1986.

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