產(chǎn)品名稱 |
Tacaribe virus |
商品貨號 |
B206709 |
Classification |
Arenaviridae, Arenavirus, New World Arenaviruses |
Agent |
Tacaribe virus |
Strain |
TRVL 11573 |
Common Name |
Tacaribe virus |
Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Isolation |
derived from existing strain |
Product Format |
frozen |
Storage Conditions |
-70°C or colder
(or colder) |
Effect on Host |
Yes, in vitro effects: Cytopathic effects, cell rounding followed by degeneration of the rounding cells CPE, rounding of cells |
Growth Medium |
Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 98%; fetal bovine serum, 2%
|
Recommended Host |
Vero cells (ATCC CCL-81) |
Growth Conditions |
Temperature: 37°C
Incubation: 2-8 days; 5% CO2 in air atmosphere is recommended; For best results cells should be 24 to 48 hours old and 70?80% confluent (not 100% confluent). |
Cryopreservation |
Storage temperature: -70°C |
Name of Depositor |
ATCC, WG Downs |
Special Collection |
NCRR Contract |
Source |
derived from existing strain |
References |
Downs WG. . Am. J. Trop. Med. Hyg. 12: 640, 1963.
i. c. inoculation
i. c. or i. p. inoculation
or colder
This is tissue culture adapted from mouse preparation ATCC VR-114 which was isolated in 1956, Port of Spain, British West Indies, from brain of bat [Artibeus literatus palmarum].
y. s. inoculation
Can be passed serially (i.c.) in adult mice, animals become ill in 5-7 days and die within 24 hours with evidence of CNS involvement. The natural clinical illness in bats resembles that produced by rabies virus. Antigenic relationship to Junin virus of Argentinian and to Machupo virus of Bolivian hemorrhagic fevers has been demonstrated by means of the CF test.
or colder
|