Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.7, 1.35, 0.94, 0.26; HindIII--6.0; EcoRI/HindIII--2.7, 1.3, 0.9, 0.7. Codominant segregation demonstrated in 55 3-generation families for the RsaI RFLP. RFLPs were observed under normal hybridization and wash stringencies. Enzyme(s) not detecting polymorphism: BamHI, BglII, EcoRI, HindIII, PstI, PvuII, MspI. |