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in another host, produces protein dihydroorotate dehydrogenase

Construct size (kb): 6.929999828338623

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pFL61
Intact vector size: 5.425
Type of vector: plasmid
Vector end: BstXI
Vector end: BstXI
Cloning sites: NotI
Polylinker sites: EcoRI BanII SacI Asp718 KpnI AvaI SmaI XmaI BamHI XbaI
Construction: pUC19
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, URA3
replicon: pMB1, 2 micron
Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: DHODH
Gene name: dihydroorotate dehydrogenase
Contains complete coding sequence?: Y
Localization: 16 q22
Tissue: HeLa D98-H2 cell line
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 1.5
Cross references: DNA Seq. Acc.: M94065

Insert lengths(kb): 1.5

Tissue: HeLa D98-H2 cell line

Gene product: dihydroorotate dehydrogenase [DHODH]

Target Gene: dihydroorotate dehydrogenase

Restriction digests of the clone give the following sizes (kb): NotI--5.5, 1.5; BamHI--4.6, 2.3; BglI--3.6; 1.9, 1.1, 0.4; PvuII--4.8, 2.1; ClaI--4.2, 2.35, 0.5.

The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--1.42; BglI--0.87, 1.25; ClaI--0.83; PstI--0.14, 0.29, 0.71; PvuII--1.06; SphI--1.3.

Contains the complete coding sequence (nt 1-1191).

Can complement ura1 mutants in Saccharomyces cerevisiae.

Detects a unique 1.6 kb transcript in humans.