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lambdaF1
lambdaF1
規(guī)格:
貨期:
編號(hào):B208068
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 lambdaF1
商品貨號(hào) B208068
Designations lambdaF1
GenBank Number

M12949

Species Homo sapiens, human
Depositors M Lefranc, TH Rabbitts
Vector
Construct size (kb): 45.0
Insert
DNA: genomic
Insert lengths(kb): 15.5
Tissue: F8 cell line (T-prolymphocytic leukemia)
Gene product: T cell receptor, gamma, joining segment JP [TCRGJ1]
Insert Size (kb) 15.5
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried bacteria-free lysate
Comments
Restriction digests of the clone give the following sizes (kb): SmaI-->23.0, 9.2, 1.7; XbaI--20.0, 13.5, 9.2, 4.6; SacI--20.0, 9.2, 5.0 (doublet), 3.0, 1.9, 1.7; XhoI--20.0, 16.0, 9.2, 2.0; PvuI-->23.0, 12.0, 8.2.
The insert contains the following restriction sites (approximate kb from the 5' end): SacI--2.9, 4.5, 9.1, 13.6, 15.5 (polylinker).
Contains the pseudogene Vgamma1 and a non-productive rearrangement of Vgamma2 and JgammaP. The insert begins approximately 5.0 kb upstream of pseudogene Vgamma1 and extends 1.6 kb beyond Jgamma1.
Additional GenBank/EMBL accession numbers: M12951, M13429, M12960, X03434.
References

LeFranc MP, et al. Diversity and rearrangement of the human T cell rearranging gamma genes: nine germ-line variable genes belonging to two subgroups. Cell 45: 237-246, 1986. PubMed: 2938743

Marie-Paule Lefranc, personal communication

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