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pRLalpha1I3/23J
pRLalpha1I3/23J
規(guī)格:
貨期:
編號:B208358
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pRLalpha1I3/23J
商品貨號 B208358
Designations pRLalpha1I3/23J
GenBank Number

J03552

Species Rattus norvegicus, rat
Depositors GH Fey
Vector
Construct size (kb): 7.599999904632568
Insert
DNA: cDNA
Insert lengths(kb): 4.599999904632568
Tissue: liver, male, acute phase
Gene product: alpha-1-inhibitor III, group 1 [A1I3A]
Insert Size (kb) 4.600
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
The vector does not have convenient restriction sites close to the insert. A BamHI digest yields 3 fragments from the insert, 5' 0.7, 0.575, 3.5 kb 3'. The end fragments contain vector sequences, and the vector band is about 3 kb.
The insert contains the following restriction sites (nucleotide position according to GenBank J03552): BamHI--583, 1158; EcoRI--746, 3814.
Restriction digests of the clone give the following sizes (kb): EcoRI--4.6, 3.0; HindIII--3.0, 2.8, 1.75, 0.4; XbaI--uncut; PstI--3.0, 2.0, 1.3, 0.9, 0.4; BamHI--3.4, 2.9, 0.7, 0.6.
The insert contains the complete coding sequence, which extends from nucleotide 80 to 4513. The signal peptide is encoded from 80 to 151.
References

Braciak TA, et al. Sequence and acute phase regulation of rat alpha1-inhibitor III messenger RNA. J. Biol. Chem. 263: 3999-4012, 1988. PubMed: 2831216

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