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熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > Microvascular Endothelial Cell Growth Kit-VEGF
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  • 0574-87157013
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  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
Microvascular Endothelial Cell Growth Kit-VEGF
Microvascular Endothelial Cell Growth Kit-VEGF
規(guī)格:
貨期:
編號:B208382
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板 



  
    

      產(chǎn)品名稱
      Microvascular Endothelial Cell Growth Kit-VEGF
    

    

      商品貨號
      B208382
    

      

      Biosafety Level
      1 




Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

    

      

      Product Format
      frozen 1 kit
    

      

      Storage Conditions
      -20°C (or -70°C for long-term storage)
    

      

      Comments
      
The Microvascular Endothelial Cell Growth Kit-VEGF contains components that when added to Vascular Cell Basal Medium (ATCC PCS-100-030) create a complete, low serum culture environment for microvascular endothelial cells isolated from normal human tissue. The final concentration for each component in complete endothelial growth medium is as follows:


    
    
  • rh VEGF: 5 ng/ml
    • 
    
  • rh EGF: 5 ng/ml
    • 
    
  • rh FGF basic: 5 ng/ml
    • 
    
  • rh IGF-1: 15 ng/ml
    • 
    
  • L-glutamine: 10 mM
    • 
    
  • Heparin sulfate: 0.75 Units/ml
    • 
    
  • Hydrocortisone: 1 µg/ml
    • 
    
  • Ascorbic acid: 50 µg/ml
    • 
    
  • Fetal bovine serum: 5% 
    • 




    

      

      Complete Growth Medium
      
    
    
  1. Obtain one growth kit from the freezer; make sure that the caps of all components are tight.
    2. 
    
  2. Thaw the components of the growth kit just prior to adding them to the basal medium. It is necessary to warm the L-glutamine component in a 37°C water bath and shake to dissolve any precipitates, prior to adding to the basal medium.
    3. 
    
  3. Obtain one bottle of Vascular Cell Basal Medium (475 mL) from cold storage.
    4. 
    
  4. Decontaminate the external surfaces of all growth kit component vials and the basal medium bottle by spraying them with 70% ethanol.
    5. 
    
  5. Using aseptic technique, and working in a laminar flow hood or biosafety cabinet transfer the volume of each growth kit component, as indicated in Table 1 or 2, to the bottle of basal medium using a separate sterile pipette for each transfer.
    6. 
    
    
    
    Table 1. If using the Microvascular Endothelial Cell Growth Kit-BBE (ATCC® PCS-110-040), add the indicated volume for each component:  
    
    
    
    
    
        
            
    
                 
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
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      Volume
      
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    Volume
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
     
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    		
    
    
      
    
      Sterility Tests
      
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    Final Concentration
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
     
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    		
    
    
      
    
      Functional Tests
      
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    Bovine Brain Extract (BBE)
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
     
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    		
    
    
      
    
      C of A
      
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    1.0 mL
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
     
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    		
    
    
        

    

						
					
					
    
							
    
							  
							  
							    
    
							      梅經(jīng)理
							      17280875617
							      1438578920
							    
    
							    
    
							      胡經(jīng)理
							      13345964880
							      2438244627
							    
    
							    
    
							      周經(jīng)理
							      17757487661
							      1296385441
							    
    
							    
    
							      于經(jīng)理
							      18067160830
							      2088210172
							    
    
							    
    
							      沈經(jīng)理
							      19548299266
							      2662369050
							    
    
							    
    
							      李經(jīng)理
							      13626845108
							      972239479
							    
    
							    
							      
							
    
					
    
				
				
    
					
				
    
			
			
		
		
    
	
  
    
	







彰武县|
舟山市|
天长市|
定南县|
将乐县|
余庆县|
辽中县|
比如县|
徐汇区|
九龙坡区|
黑水县|
太保市|
兴和县|
新和县|
绥芬河市|
阜宁县|
北宁市|
蓝田县|
宾阳县|
澄江县|
祁东县|
庐江县|
桂林市|
徐州市|
托克托县|
隆昌县|
博乐市|
突泉县|
澄江县|
柳江县|
中西区|
东方市|
浦江县|
辽阳县|
那坡县|
忻州市|
霸州市|
咸宁市|
常宁市|
深圳市|
雷山县|


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