亚洲精品无码成人片久久不卡,亚洲自偷自偷在线传媒,亚洲精品在线,久久无码精品九号,欧洲av人人爽爽,亚洲精品蜜桃久久久久久,亚洲精品久久午夜无码专区电影,辽宁少妇高潮45分钟,少妇高清性色生活片,无码八少妇久久

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > pBC12BI [4053B]
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • [email protected]
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
pBC12BI [4053B]
pBC12BI [4053B]
規(guī)格:
貨期:
編號:B208488
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pBC12BI [4053B]
商品貨號 B208488
Designations pBC12BI [4053B]
Depositors Hoffmann-La Roche Ltd., PC Watkins, Hoffmann-La Roche Ltd.
U.S. Patent
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Biosafety Level 1
Vector Information
Size (kb): 4.0700001716613770
Vector: pBC12BI (plasmid)
Promoters: Promoter RSV LTR
Construction: pXF3, pBC81delta4, SV40
Marker(s):ampR
Construct size (kb): 4.099999904632568
Features: marker(s): ampR
promoter: RSV LTR
replicon: pMB1, SV40
terminator: rat insulin II
enhancer: RSV LTR
Applications
contains sequence preproinsulin 2
expression vector
shuttle vector
vector permitting construction of fusion proteins
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--4.1; EcoRI--2.8, 0.78, 0.44; HindIII--4.1; BstEII--4.1.
This was constructed from pXF3 (pBR322 minus the poison sequence), a SV40 ori, and a rat preproinsulin genomic sequence under the control of RSV LTR (pBC81delta4 cut at BgIII and BalI and inserted at the BamHI and ClaI sites).
cDNA sequences with a translation initiation codon are usually inserted between the unique HindIII and BamHI sites (5' to 3'). Polyadenylation signals (and possibly carboxy terminal amino acids) are contributed by the preproinsulin gene.
Genomic sequences are normally inserted between unique HindIII and SmaI sites (5' to 3'). This deletes the preproinsulin gene from the vector.
cDNA clones without an initiation codon may be cloned in frame into the unique BamHI site 3' to the AUG of the rat preproinsulin gene. This results in 5 - 6 additional amino terminal amino acids from the insulin signal peptide.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Cullen BR. Use of eukaryotic expression technology in the functional analysis of cloned genes. Methods Enzymol. 152: 684-704, 1987. PubMed: 3657593

Shipped frozen
Shipping Information Distributed: freeze-dried
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
盘山县| 桃园县| 西吉县| 红桥区| 广德县| 常宁市| 临颍县| 永城市| 西和县| 亳州市| 黄龙县| 胶南市| 沙湾县| 普安县| 五指山市| 肃南| 遵化市| 上高县| 焉耆| 永善县| 定南县| 长春市| 互助| 合江县| 察隅县| 天气| 滨海县| 潞城市| 宜春市| 嫩江县| 昭觉县| 汝阳县| 潍坊市| 普格县| 南安市| 花莲市| 舞阳县| 峨眉山市| 思南县| 垫江县| 英吉沙县|