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pFEN207
pFEN207
規(guī)格:
貨期:
編號:B208890
品牌:Mingzhoubio

標準菌株
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DNA
RNA

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產(chǎn)品名稱 pFEN207
商品貨號 B208890
Designations pFEN207
GenBank Number

M64618

Species Chlamydia trachomatis
Depositors Rocky Mountain Laboratory, FE Nano, Rocky Mountain Laboratory
Applications
produces protein 3-deoxy-D-manno-octulosonic acid transferase (Kdo transferase)
Vector
Construct size (kb): 9.2
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC8
Intact vector size: 2.700
Type of vector: plasmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI SmaI XmaI BamHI SalI AccI HincII PstI HindIII
Polylinker sites: EcoRI SmaI XmaI BamHI SalI AccI HincII PstI HindIII
Construction: pBR322, Escherichia coli lacZalpha, M13mp8
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac
replicon: pMB1
terminator: none
Cross references:
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Chlamydia trachomatis
Gene symbol: gseA
Gene name: 3-deoxy-D-manno-octulosonic acid transferase (Kdo
transferase)
Contains complete coding sequence?: Y
Strain: LGV-434 (serovar L2)
Type of DNA: genomic
Insert end: Sau3AI
Insert end: Sau3AI
Insert size (kb): 6.5
Cross references: DNA Seq. Acc.: M64618
Insert lengths(kb): 6.5
Gene product: 3-deoxy-D-manno-octulosonic acid transferase (Kdo transferase) [gseA]
Target Gene: 3-deoxy-D-manno-octulosonic acid transferase (Kdo transferase)
Insert Size (kb) 6.5
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.2, 2.8; EcoRI/HindIII--2.7, 1.35 (doublet), 0.84 (triplet), 0.64, 0.36, 0.27; ScaI/SacII--4.6, 2.4, 1.4, 0.96.
Expresses the genus-specific lipopolysaccharide epitope of Chlamydia.
The insert contains at least 2 open reading frames. The 1.4 kb ScaI/SacII fragment contains the complete coding sequence of gseA and has been sequenced.
Classification Chlamydiaceae, Chlamydia
References

Nano FE, Caldwell HD. Recombinant clones of Chlamydia trachomatis lipopolysaccharide. US Patent 5,075,228 dated Dec 24 1991

Nano FE, Caldwell HD. Expression of the Chlamydial genus-specific lipopolysaccharide epitope in Escherichia coli. Science 228: 742-744, 1985. PubMed: 2581315

Belunis CJ, et al. A novel 3-deoxy-D-manno-octulosonic acid transferase from Chlamydia trachomatis required for expression of the genus-specfic epitope. J. Biol. Chem. 267: 18702-18707, 1992. PubMed: 1382060

Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
梅經(jīng)理 17280875617 1438578920
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周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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