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pobIT [OBIT]
pobIT [OBIT]
規(guī)格:
貨期:
編號:B208894
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pobIT [OBIT]
商品貨號 B208894
Designations pobIT [OBIT]
Species Mus musculus, mouse
Depositors ML Lane
Vector
Construct size (kb): 5.199999809265137
Insert
DNA: genomic
Insert lengths(kb): 1.200000047683716
Tissue: NIH 3T3 cells transformed by DNA from S49 lymphoma
Gene product: T lymphoma oncogene [Tlm]
Insert Size (kb) 1.200
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): HindIII/AVAI--3.0, 2.5; HindIII--5.4; AvaI--5.4; BamHI--uncut; AccI--4.0, 1.6.
pobIT contains 5' flanking sequences and is a single copy probe.
This probe detects genomic BamHI fragments of 18 and 8.7 kb in normal NIH 3T3 cells and transformed cell lines. The 8.7 kb band corresponds to the clone.
This probe detects genomic HindIII fragments of approximately 30 kb and 6.2 kb in normal 3T3 cells and transformed cell lines.
Classification Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

Lane MA, Tobin MB. Genomic sequence of the mouse oncogene tlm. Nucleic Acids Res. 18: 3410, 1990. PubMed: 2356136

Lane MA, et al. Isolation and characterization of a stage-specific transforming gene, Tlym-I, from T-cell lymphomas. Proc. Natl. Acad. Sci. USA 81: 2227-2231, 1984. PubMed: 6326114

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