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pSM257
pSM257
規(guī)格:
貨期:
編號:B208897
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pSM257
商品貨號 B208897
Designations pSM257
GenBank Number

M25247

Species Pseudomonas sp.
Depositors G Grandi
Applications
produces protein isoamylase
Vector
Construct size (kb): 6.0
Insert
DNA: genomic
Insert lengths(kb): 3.339999914169312
Gene product: isoamylase [iam]
Target Gene: isoamylase
Insert Size (kb) 3.340
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--4.3, 1.7; HindIII--6.1; PstI--6.0; BamHI--6.1.
This clone was previously deposited as ATCC 67474.
The insert contains the following restriction sites (nt position according to the published sequence): AatII--618; BamHI--688; EcoRI--973.
Secretes isoamylase regulated by a promoter in the insert. The recombinant protein is recognized by anti-SMP1 isoamylase antibodies.
References

Tognoni A, et al. Cloning and nucleotide sequence of the isoamylase gene from a strain of Pseudomonas sp.. J. Gen. Microbiol. 135: 37-45, 1989. PubMed: 2778432

Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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