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Distribution host: Escherichia coli HB101 (ATCC 33694)

Size (kb): 8.3999996185302730

DESCRIPTION OF VECTOR:
Intact vector size: 8.400
Type of vector: plasmid
Cloning sites:
Polylinker sites:
Construction: YRp14
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, URA3, SUP11
replicon: pMB1, ARS1

Vector: YRp14-trp1delta63 (plasmid)

Construction: YRp14

Marker(s):URA3,ampR,SUP11

Construct size (kb): 8.399999618530273

Features: marker(s): ampR, URA3, SUP11
replicon: pMB1, ARS1

vector for other uses

Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--6.0, 1.6, 0.6; HindIII--8.4; BamHI--8.4; PstI--3.7, 3.4, 1.3; BglII--7.4, 1.1.

A marker addition/transplacement vector.

Non-integrants grow poorly selecting for Ura3+ (pinpoints at 5 days) while integrants grow well because the SUP11 copy number is maintained at one. If there are no integrants on a transformation plate, streak onto -URA plates and pick papillae.

Constructed by inserting the equivalent of 2 genomic EcoRI/HindIII fragments (1.5 kb, centromere proximal and 0.8 kb, 3' end) from the TRP1 region into YRp14.

Represents the deletion of a 0.6 kb EcoRI/HindIII fragment containing the promoter and the 5' portion of the gene.

Temperature: 37.0°C