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pZilch
pZilch
規(guī)格:
貨期:
編號:B209044
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pZilch
商品貨號 B209044
Designations pZilch
Depositors C Hovens
Biosafety Level 1
Vector Information
Size (kb): 6.0770001411438000
Vector: pZilch (phagemid)
Promoters: Promoter CMV
Construction: pCATCH
Marker(s):ampR
Construct size (kb): 6.0770001411438
Features: marker(s): ampR
promoter: CMV
promoter for in vitro transcription: SP6
promoter for in vitro transcription: T7
replicon: M13
replicon: SV40 ori
replicon: pMB1
replicon: polyoma
MCS: BamHI...XhoI
MCS: BglII...SalI
terminator: SV40
coding sequence: mutant pheS gene
epitope tag: FLAG
Applications
encodes removable tag for protein isolation
expression vector
vector permitting construction of fusion proteins
vector permitting positive selection for inserts
vector permitting production of single-stranded DNA
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.1; EcoRI--6.1.
The vector was contructed by inserting a 1185 bp SmaI/BamHI fragment from pKSS encompassing the mutant pheS allele into the pCATCH and modified further to generate polylinker and to incorporate Kozak sequence and initiation ATG codon.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Schneider S, et al. An epitope tagged mammalian/prokaryotic expression vector with positive selection of cloned inserts. Gene 197: 337-341, 1997. PubMed: 9332383

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梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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