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pGB42
pGB42
規(guī)格:
貨期:
編號(hào):B209222
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pGB42
商品貨號(hào) B209222
Designations pGB42
GenBank Number

M11080

Species Mus musculus, mouse
Depositors Genzyme Corp., G Barsomian, Genzyme Corp.
Applications
in another host, produces protein glucosidase, beta; acid Glucosidase, beta, acid, glucocerebrosidase
Vector
Construct size (kb): 6.0
Insert
DNA: cDNA
Insert lengths(kb): 1.799999952316284
Gene product: dihydrofolate reductase [GBA]
Target Gene: glucosidase, beta; acid
Insert Size (kb) 1.800
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.0; BglII--6.0; HindIII--6.0.
The glucocerebrosidase insert contains the following restriction sites (approximate kb from the 5' end): HindIII--0.83; PstI--0.27, 0.56; SalI--1.25; BamHI--1.48.
Contains the complete coding sequence for glucocerebrosidase (nt 6-1556), plus 290 nt at the 3' end. Corresponds approximately to nt 490-2336 of the sequence record, with a modification at the 5' end from CCCACATGG to CCACCATGG.
The vector was derived from pSV2-dhfr by adding the adenovirus promoter and removing the poison sequence from the pBR322 portion.
Permits bicistronic expression, with glucocerebrosidase being translated before dhfr, resulting in greater expression of the insert sequence relative to dhfr.
The order of the major features in the plasmid is: pMB1 ori - SV40 enhancer - Adenovirus major late promoter - XhoI - BglII - glucocerebrosidase insert - dihydrofolate reductase - SV40 polyadenylation - ampR.
Classification Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

Rassmussen J, et al. Enzymatically active recombinant glucocerebrosidase. US Patent 5,236,838 dated Aug 17 1993

Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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