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RA-1.2
RA-1.2
規(guī)格:
貨期:
編號(hào):B209313
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 RA-1.2
商品貨號(hào) B209313
Designations RA-1.2
GenBank Number

U31346

Species Homo sapiens, human
Depositors H Menard
Applications
This clone expresses a fusion protein of GST and a truncated calpastatin.
Vector
Construct size (kb): 4.0
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC19
Intact vector size: 2.686
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI
HindIII
Polylinker sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI
HindIII
Construction: pUC71K
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ', <-
MCS: EcoRI...HindIII, ->
promoter: lac, <-
replicon: pMB1, <-
marker(s): ampR, <-
Cross references: DNA Seq. Acc.: X02514
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CAST
Gene name: calpastatin
Contains complete coding sequence?: N
Chromosome: 5; Localization: 5 q15-q21
Tissue: placenta
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.323
Cross references: DNA Seq. Acc.: U31346
Nucleotides 1-1323 of the insert correspond to
nucleotides 1-1323 of U31346.
Insert lengths(kb): 1.322999954223633
Tissue: placenta
Gene product: calpastatin [CAST]
Insert Size (kb) 1.323
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 1.3; BamHI--4.0; HindIII--3.0, 0.65, 0.35; PstI--3.1, 0.9; EcoRI/PstI--2.7, 0.9, 0.5.
The insert contains the following restriction sites (approximate kb from the 5' end): HindIII--0.30,0.87,1.15; PstI 0.42.
This clone expresses a fusion protein of GST and a truncated calpastatin. This calpastatin begins at nt1677 of D16217 and continues to the poly(A) tail, but the 3' UTR is different.
References

Despres N, et al. Detection and expression of a cDNA clone that encodes a polypeptide containing two inhibitory domains of human calpastatin and its recognition by rheumatoid arthritis sera. J. Clin. Invest. 95: 1891-1896, 1995. PubMed: 7706496

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