亚洲精品无码成人片久久不卡,亚洲自偷自偷在线传媒,亚洲精品在线,久久无码精品九号,欧洲av人人爽爽,亚洲精品蜜桃久久久久久,亚洲精品久久午夜无码专区电影,辽宁少妇高潮45分钟,少妇高清性色生活片,无码八少妇久久

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > Giardia intestinalis (Lambl) Alexeieff
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • [email protected]
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
Giardia intestinalis (Lambl) Alexeieff
Giardia intestinalis (Lambl) Alexeieff
規(guī)格:
貨期:
編號:B209382
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱 Giardia intestinalis (Lambl) Alexeieff
商品貨號 B209382
Strain Designations AB
Application
Enteric Research
Food and waterborne pathogen research
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation Unknown
Product Format frozen
Storage Conditions Frozen Cultures:
-70°C for 1 week; liquid N2 vapor for long term storage

Freeze-dried Cultures:
2-8°C

Live Cultures:
See Protocols section for handling information
Type Strain no
Comments
Strain variation in excretory/secretory products and antigens
RFLP analysis of DNA
Medium ATCC® Medium 2695: Keister's Modified TYI-S-33
ATCC® Medium 2155: LYI Giardia Medium (filtered)
Growth Conditions Temperature: 35°C
Culture System: Axenic
Cryopreservation Harvest and Preservation
  1. Harvest cells from a culture that is at or near peak density. To detach cells from the wall of the culture tubes place on ice for 10 minutes. Invert tubes several times until the majority of the cells are in suspension. Centrifuge tubes at 800 x g for 5 minutes.
  2. Adjust the concentration of cells to 2 x 107/mL in fresh medium.
  3. Before centrifuging prepare a 24% (v/v) solution of sterile DMSO in fresh medium containing 8% (w/v) sucrose. The solution is prepared as follows:
    1. Add 1.05 g sucrose to 10 mL of fresh medium and filter sterilize through a 0.2 µm filter;
    2. Add 2.4 mL of DMSO to an ice cold 20 x 150 mm screw-capped test tube;
    3. Place the tube on ice and allow the DMSO to solidify (~5 min) and then add 7.6 mL of ice cold medium prepared in step 3a. The final concentration will be 24% (v/v) DMSO and 8% (w/v) sucrose;
    4. Invert several times to dissolve the DMSO;
    5. Allow to warm to room temperature.
  4. Mix the cell preparation and the cryoprotective agent, prepared in step 3, in equal portions. Thus, the final concentration will equal 12% (v/v) DMSO + 4% sucrose (w/v) and 107 cells/mL. The time from the mixing of the cell preparation and DMSO stock solution to the start of the freezing process should be no less than 15 min and no longer than 30 min.
  5. Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
  6. Place the vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If the freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through the heat of fusion. At -40°C plunge into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus. Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen. (The cooling rate in this apparatus is approximately -1°C/min.)
  7. The frozen preparations should be stored in either the vapor or liquid phase of a nitrogen refrigerator. Frozen preparations stored below -130°C are stabile indefinitely. Those stored at temperatures above -130°C are progressively less stabile as the storage temperature is elevated.
  8. To establish a culture from the frozen state place an ampule in a water bath set at 35°C. Immerse the vial just to a level just above the surface of the frozen material. Do not agitate the vial.
  9. Immediately after thawing, do not leave in the water bath, aseptically remove the contents of the ampule and inoculate a 16 x 125 mm screw-capped test tube containing 13 mL ATCC Medium 2695.
  10. Incubate the culture on a 15º horizontal slant at 35°C.
Name of Depositor TE Nash
Special Collection NCRR Contract
References

Nash TE, et al. Restriction-endonuclease analysis of DNA from 15 Giardia isolates obtained from humans and animals. J. Infect. Dis. 152: 64-73, 1985. PubMed: 2409186

Nash TE, Keister DB. Differences in excretory-secretory products and surface antigens among 19 isolates of Giardia. J. Infect. Dis. 152: 1166-1171, 1985. PubMed: 4067331

梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479
文登市| 日喀则市| 库伦旗| 原平市| 南投县| 宜城市| 林口县| 绥宁县| 深圳市| 日喀则市| 墨脱县| 正阳县| 廉江市| 建始县| 高平市| 黎川县| 庄浪县| 博湖县| 溆浦县| 广河县| 上思县| 纳雍县| 缙云县| 麻栗坡县| 永济市| 九江市| 慈溪市| 西贡区| 大理市| 揭西县| 泌阳县| 莱州市| 峨眉山市| 罗城| 独山县| 沙河市| 桓台县| 南和县| 凌云县| 石屏县| 大悟县|