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36B4
36B4
規(guī)格:
貨期:
編號:B209488
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 36B4
商品貨號 B209488
Designations 36B4
Species Homo sapiens, human
Depositors P Chambon
Applications
Used as a control in RNase protection experiments to study the regulation by estradiol of transcription.
Vector
Construct size (kb): 5.369999885559082
Insert
DNA: cDNA
Insert lengths(kb): 1.067999958992004
Tissue: breast cancer MCF-7 cell line
Gene product: ribosomal phosphoprotein P0(acidic ribosomal protein P0 (L10E)) [RPP0*]
Insert Size (kb) 1.068
Media ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--5.4; PstI--4.4, 0.7, 0.2, 0.1; HindIII--5.4; BamHI--5.4; SalI--5.4.
The insert contains the following restriction sites (nt from one end): PstI--708, 928.
Expressed constitutively, and not stimulated by estradiol. Used as a control in RNase protection experiments to study the regulation by estradiol of transcription.
References

Masiakowski P, et al. Cloning of cDNA sequences of hormone-regulated genes from the MCF-7 human breast cancer cell line. Nucleic Acids Res. 10: 7895-7903, 1982. PubMed: 6897676

Laborda J. 36B4 cDNA used as an estradiol-independent mRNA control is the cDNA for human acidic ribosomal phosphoprotein PO. Nucleic Acids Res. 19: 3998, 1991. PubMed: 1861990

Brown AM, et al. Activation of pS2 gene transcription is a primary response to estrogen in the human breast cancer cell line MCF-7. Proc. Natl. Acad. Sci. USA 81: 6344-6348, 1984. PubMed: 6593703

Rio MC, et al. Specific expression of the pS2 gene in subclasses of breast cancers in comparison with expression of the estrogen and progesterone receptors and the oncogene ERBB2. Proc. Natl. Acad. Sci. USA 84: 9243-9247, 1987. PubMed: 3321071

Pierre Chambon, personal communication

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