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Distribution host: Escherichia coli HB101 (ATCC 33694)

Size (kb): 13.5000000000000000

DESCRIPTION OF VECTOR:
Intact vector size: 13.500
Type of vector: plasmid
Cloning sites: EcoRI SstI SmaI XbaI SalI HindIII BamHI
Polylinker sites:
Construction: pBIN19, pBI101, pBIB-HYG
Host range: Rhizobium radiobacter; higher plants; Escherichia coli
Features (with orientation and position when available):
marker(s): kanR
replicon: RK2

Vector: pGPTV-KAN (plasmid)

Construction: pBIN19, pBI101, pBIB-HYG

Marker(s):kanR

Construct size (kb): 13.5

Features: marker(s): kanR
replicon: RK2

shuttle vector

Restriction digests of the clone give the following sizes (kb): EcoRI--13.6; BglII--8.2, 3.2, 2.1; HindIII--13.6.

Vector contains the following restriction sites (approximate kb from the HindIII site): BamHI--1.4; EcoRI--11.4; SstI--11.7.

Binary vector with plant selectable marker near the T-DNA left border and a glucuronidase (uidA) reporter gene. The marker and uidA are divergently transcribed, and can be easily removed or replaced.

The order of the major features of this plasmid is: EcoRI - pAnos - SstI - uidA - SmaI - XbaI - SalI - HindIII - Pnos - BglII - nptII - BamHI - pAg7 - T-DNA - BglII - RK2 ori - kanR.

Temperature: 37.0°C