Vector Information |
Size (kb): 6.7069997787475590 DESCRIPTION OF VECTOR: Intact vector size: 6.707 Type of vector: plasmid Cloning sites: XbaI SpeI BamHI SmaI PstI HindIII SalI XhoI Polylinker sites: XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI SalI XhoI Construction: pRS415, GAL1 promoter Host range: Saccharomyces cerevisiae; Escherichia coli Features (with orientation and position when available): marker(s): LEU2, ->, 200-2446 terminator: CYC1, ->, 3128-3388 MCS: XhoI...XbaI, ->, 3388-3451 promoter for expression: GAL1, <-, 3452-3912 marker(s): ampR, <-, 5159-6089 centromere: CEN6/ARSH4, ->, 6193-6707 Vector: p415 GAL1 (plasmid) Promoters: Promoter for expression GAL1 Construction: pRS415, GAL1 promoter Marker(s):LEU2,ampR Construct size (kb): 6.706999778747559 Features: marker(s): LEU2 marker(s): ampR promoter for expression: GAL1 MCS: XhoI...XbaI terminator: CYC1 centromere: CEN6/ARSH4 |
Comments |
Expression from the galactokinase (GAL1) promoter is tightly repressed by glucose and is strongly induced by galactose. Low copy number shuttle expression vector. One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon. |