亚洲精品无码成人片久久不卡,亚洲自偷自偷在线传媒,亚洲精品在线,久久无码精品九号,欧洲av人人爽爽,亚洲精品蜜桃久久久久久,亚洲精品久久午夜无码专区电影,辽宁少妇高潮45分钟,少妇高清性色生活片,无码八少妇久久

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > Babesia microti (Franca) Reichenow
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • [email protected]
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
Babesia microti (Franca) Reichenow
Babesia microti (Franca) Reichenow
規(guī)格:
貨期:
編號:B209688
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Babesia microti (Franca) Reichenow
商品貨號 B209688
Strain Designations GI (Ingram strain)
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation Blood, human babesiosis, Nantucket, MA, 1983
Product Format frozen
Storage Conditions Frozen Cultures:
-70°C for 1 week; liquid N2 vapor for long term storage

Freeze-dried Cultures:
2-8°C

Live Cultures:
See Protocols section for handling information
Comments Genome sequenced strain
Growth Conditions Culture System: In vivo, Golden Syrian hamster
Cryopreservation Reagents
Alsever's Solution
NaCl, 4.2 g
Na3citrate•2H2O, 8.0 g
Glucose, 20.5 g
Glass distilled H2O to 1.0 L
*Dissolve components in glass distilled H2O, adjust the pH to 6.1 with 10% (w/v) citric acid and filter sterilize. The solution can be obtained from Sigma-Aldrich (cat# A3551).

Harvest and Preservation
  1. Prepare a 30% (v/v) sterile glycerol solution in Alsever's solution.
  2. Draw approximately 0.5 mL of anticoagulant solution (Yaeger's or heparin, etc.) into a syringe and move it back and forth over the length of the syringe, several times.  Remove all air bubbles.  Draw blood by cardiac puncture into the syringe from a host animal that has reached or is near peak parasitemia.  If clotting occurs during extraction of blood, insufficient heparin was used.  
  3. Mix the heparinized blood with the 30% glycerol solution in a 2:1 ratio.  If any clotting has occurred do not use.  After mixing, the final concentration of cryoprotectant solution will be 10% (v/v).  The mixture should be placed in a 4°C ice bath.  The time from the mixing of the cell preparation and glycerol stock solution before the freezing process is begun should be no less than 15 min and no longer than 30 min.
  4. Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryovials (special plastic vials for cryopreservation).  Filled ampules should be placed in a 4°C ice bath.  Do not immerse ampules to the level of the vial cap.
  5. Plunge ampules from 4°C into liquid nitrogen.  The frozen preparations may be stored in a mechanical freezer until needed, however storage in either the vapor or liquid phase of a nitrogen refrigerator is recommended for the longest viability.
  6. To thaw a frozen ampule, place in a 35°C water bath, until thawed (2-3 min).  Immerse the ampule just sufficient to cover the frozen material.  Do not agitate the ampule.
  7. Immediately after thawing, aseptically remove the contents of the ampule with a syringe and inoculate an uninfected hamster.  Follow the protocol for maintenance in vivo.  The course of infection may be longer or shorter than usual depending on recovery of the parasite from the frozen state.
Name of Depositor C Ben Mamoun
Chain of Custody ATCC <-- C Ben Mamoun
References

Gray J, et al. Transmission studies of Babesia microti in Ixodes ricinus ticks and gerbils. J Clin Microbiol 40(4): 1259-1263, 2002. PubMed: 11923342

Piesman J, et al. Development of Babesia microti sporozoites in adult Ixodes dammini. Int J Parasitol 16(4): 381-385, 1986. PubMed: 3744675

Ryan R, et al. Diagnosis of babesiosis using an immunoblot serologic test. Clin Diagn Lab Immunol 8(6): 1177-1180, 2001. PubMed: 11687460

Wilson M, et al. Development of droplet digital PCR for the detection of Babesia microti and Babesia duncani. Exp. Parasitol. 149: 24-31, 2015. PubMed: 25500215

Cross References

Nucleotide (GenBank) : AF510194.1 Babesia microti strain GI 18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence

Nucleotide (GenBank) : AF231348.1 Babesia microti strain GI nuclear small subunit ribosomal RNA gene, partial sequence

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
太白县| 教育| 长治市| 连南| 岐山县| 司法| 唐河县| 济南市| 淳安县| 罗城| 黄冈市| 武穴市| 云龙县| 蚌埠市| 仙游县| 深泽县| 泸西县| 东兰县| 全南县| 会昌县| 阳谷县| 乾安县| 岫岩| 晴隆县| 睢宁县| 韩城市| 海晏县| 额济纳旗| 二连浩特市| 福安市| 萨嘎县| 平定县| 东乌珠穆沁旗| 霍林郭勒市| 台湾省| 扎兰屯市| 仙游县| 兴义市| 高邮市| 苏州市| 抚宁县|