產(chǎn)品名稱 |
Human herpesvirus 6B |
商品貨號(hào) |
B209768 |
Classification |
Herpesviridae, Roseolovirus |
Deposited As |
Human herpesvirus 6 |
Agent |
Human herpesvirus 6B |
Strain |
SF |
Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Product Format |
frozen 1 mL per vial |
Storage Conditions |
Vapor phase of liquid nitrogen |
Images |
 |
Effect on Host |
CPE, cell enlargement and balloon-like degeneration |
Recommended Host |
Human peripheral blood mononuclear cells |
Growth Conditions |
Temperature: 37°C
Recommendations for Infection: This product is produced by co-cultivation of virus with fresh host cells. Prepare a bulk cell suspension the day of inoculation. Seed culture vessels at 5 x 105 cells per mL. Calculate the volume of virus needed to achieve an optimal MOI (e.g. 0.1) and then dilute virus in virus growth medium to prepare the virus inoculum. Add virus inoculum to culture vessels. Incubate at 37°C in a humidified 5% CO2 atmosphere.
Incubation: 7-9 days |
Name of Depositor |
JA Levy, University of California School of Medicine |
Special Collection |
NCRR Contract |
Source |
Saliva from an HIV-infected patient, San Francisco, California |
References |
Levy JA, et al. Characterization of a new strain of HHV-6 (HHV-6SF) recovered from the saliva of an HIV-infected individual. Virology 178: 113-121, 1990. PubMed: 1975142
Jay A Levy, personal communication
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