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Trypanosoma equinum Voges
Trypanosoma equinum Voges
規(guī)格:
貨期:
編號(hào):B209826
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Trypanosoma equinum Voges
商品貨號(hào) B209826
Strain Designations Wellcome
Application
Vector borne research
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Type Strain no
Comments
Catabolism of tryptophan
Loss of kinetoplast
Growth Conditions
Duration: in vivo, laboratory rat
Protocol: ATCCNO: 30018 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. Upon arrival remove the frozen ampoule from the dry ice and transfer directly to a 35C water bath. When completely thawed, aseptically remove the material with a syringe and inject 0.2 ml aliquots of the thawed contents of the ampule intraperitoneally into an adult rats known to be free of blood parasites. Draw a drop of blood from the tail of an infected rat. Add 1.0 ml of mammalian saline (0.8% saline buffered to pH 7.0) to the blood and examine a small amount microscopically. If one or more parasites are seen, proceed as follows: collect parasites into a syringe containing a buffered anticoagulant by either cardiac puncture or from the tail vein of the infected animal. Inoculate 0.2 ml of the diluted blood intraperitoneally into an adult rat known to be free of other blood parasites. Repeat the above procedures approximately every 4 days.
Subcultivation
Protocol: ATCCNO: 30018 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. Upon arrival remove the frozen ampoule from the dry ice and transfer directly to a 35C water bath. When completely thawed, aseptically remove the material with a syringe and inject 0.2 ml aliquots of the thawed contents of the ampule intraperitoneally into an adult rats known to be free of blood parasites. Draw a drop of blood from the tail of an infected rat. Add 1.0 ml of mammalian saline (0.8% saline buffered to pH 7.0) to the blood and examine a small amount microscopically. If one or more parasites are seen, proceed as follows: collect parasites into a syringe containing a buffered anticoagulant by either cardiac puncture or from the tail vein of the infected animal. Inoculate 0.2 ml of the diluted blood intraperitoneally into an adult rat known to be free of other blood parasites. Repeat the above procedures approximately every 4 days.
Name of Depositor EJ Tobie
Chain of Custody
ATCC <<--EJ Tobie<<--C.A. Hoare <<--- Instituto Oswaldo Cruz, Brazil, ca. 1943
References

. . J. Protozool. 1: 28, 1954.

Sawalhy AE, et al. Catabolism of tryptophan by Trypanosoma evansi. J. Eukaryot. Microbiol. 42: 684-690, 1995. PubMed: 8520582

Hoare CS. The loss of the kinetoplast in Trypanosomas, with special reference to Trypanosoma evansi. J. Protozool. 1: 28-33, 1954.

Cuthbertson RS. Kinetoplast DNA in Trypanosoma equinum. J. Protozool. 28: 182-188, 1981. PubMed: 7277256

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沈經(jīng)理 19548299266 2662369050
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