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Size (kb): 7.4609999656677250

DESCRIPTION OF VECTOR:
Intact vector size: 7.461
Type of vector: phagemid
Cloning sites: SpeI BamHI SmaI EcoRI EcoRV ClaI SalI XhoI
Polylinker sites: SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI SalI Xho
Construction: pRS425, CTR1-CYC1 promoter
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
marker(s): LEU2, <-
replicon: f1, <-
terminator: CYC1, <-
MCS: XhoI...SpeI, ->
promoter for expression: CTR1-CYC1, ->
replicon: pMB1
marker(s): ampR, <-
replicon: 2 micron, <-

Vector: pCu425CTR1-CYC1 (phagemid)

Promoters: Promoter for expression CTR1-CYC1

Construction: pRS425, CTR1-CYC1 promoter

Marker(s):LEU2,ampR

Construct size (kb): 7.460999965667725

Features: marker(s): LEU2
marker(s): ampR
promoter for expression: CTR1-CYC1
replicon: 2 micron
replicon: f1
replicon: pMB1
MCS: XhoI...SpeI
terminator: CYC1

cloning vector

Restriction digests of the vector give the following sizes (kb): SacI--7.5; SacI/SpeI--7.0, 0.38.

CTR3 and CTR1 are the copper transporter promoters and are repressed by the presence of copper and highly activate under copper-starvation conditions. The time course of copper-starvation activation and copper repression of a reporter gene is rapid.

component of:ATCC 87778