| 產(chǎn)品名稱 | A549 |
|---|---|
| 商品貨號 | B211337 |
| Organism | Homo sapiens, human |
| Tissue | Lung |
| Cell Type | epithelial cell (KRAS CRM) |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | Carcinoma |
| Age | 58 years |
| Gender | Male |
| Ethnicity | Caucasian |
| Applications | For use in testing and calibration in ISO 17025 accredited laboratories, to challenge assay performance, validate or compare test methods, and to establish sensitivity, linearity and specificity during assay validation or implementation. This cell line may be used as a transfection host. |
| Intended Use | Certified Reference Material produced under an ISO 17034 accredited process.
|
| Storage Conditions | liquid nitrogen vapor phase |
| Karyotype | This is a hypotriploid human cell line with the modal chromosome number of 66, occurring in 24% of cells. Cells with 64 (22%), 65, and 67 chromosome counts also occurred at relatively high frequencies; the rate with higher ploidies was low at 0.4%. There were 6 markers present in single copies in all cells. They include der(6)t(1;6) (q11;q27); ?del(6) (p23); del(11) (q21), del(2) (q11), M4 and M5. Most cells had two X and two Y chromosomes. However, one or both Y chromosomes were lost in 40% of 50 cells analyzed. Chromosomes N2 and N6 had single copies per cell; and N12 and N17 usually had 4 copies. |
| Derivation | This line was initiated in 1972 by D.J. Giard, et al. through explant culture of lung carcinomatous tissue from a 58-year-old Caucasian male. |
| Comments | Certificates of Analysis are available electronically at www.atcc.org, or by hardcopy upon request. Certified Reference Material produced under an ISO 17034 accredited process. This cell line has been tested for the KRAS mutation (p.G12S c.34G>A) Studies by M. Lieber, et al. revealed that A549 cells could synthesize lecithin with a high percentage of desaturated fatty acids utilizing the cytidine diphosphocholine pathway. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended Medium Renewal: 2 to 3 times per week |
| Cryopreservation | Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage Temperature: Liquid nitrogen vapor phase |
| STR Profile | Amelogenin: X,Y CSF1PO: 10, 12 D13S317: 11 D16S539: 11,12 D5S818: 11 D7S820: 8, 11 TH01: 8, 9.3 TPOX: 8, 11 vWA: 14 |
| Population Doubling Time | about 22 hours |
| Year of Origin | 1972 |
| References | Giard DJ, et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758 King BL, et al. Repeat expansion detection analysis of (CAG)n tracts in tumor cell lines, testicular tumors, and testicular cancer families. Cancer Res. 57: 209-214, 1997. PubMed: 9000556 Jeffers M, et al. Degradation of the Met tyrosine kinase receptor by the ubiquitin-proteasome pathway. Mol. Cell. Biol. 17: 799-808, 1997. PubMed: 9001234 Siim BG, et al. Tirapazamine-induced cytotoxicity and DNA damage in transplanted tumors: Relationship to tumor hypoxia. Cancer Res. 57: 2922-2928, 1997. PubMed: 9230202 Zhao Y, et al. Cloning and chromosomal location of a novel member of the myotonic dystrophy family of protein kinases. J. Biol. Chem. 272: 10013-10020, 1997. PubMed: 9092543 Goodrum FD, Ornelles DA. The early region 1B 55-kilodalton oncoprotein of adenovirus relieves growth restrictions imposed on viral replication by the cell cycle. J. Virol. 71: 548-561, 1997. PubMed: 8985383 Putzer BM, et al. Interleukin 12 and B7-1 costimulatory molecule expressed by an adenovirus vector act synergistically to facilitate tumor regression. Proc. Natl. Acad. Sci. USA 94: 10889-10894, 1997. PubMed: 9380730 Pulli T, et al. Cell-surface interactions of echovirus 22. J. Biol. Chem. 272: 21176-21180, 1997. PubMed: 9261123 de Caestecker MP, et al. Characterization of functional domains within Smad4/DPC4. J. Biol. Chem. 272: 13690-13696, 1997. PubMed: 9153220 Li Y, et al. Nuclear factor-kappaB is activated by hyperoxia but does not protect from cell death. J. Biol. Chem. 272: 20646-20649, 1997. PubMed: 9252381 Gao E, et al. The basic helix-loop-helix-zipper transcription factor USF1 regulates expression of the surfactant protein-A gene. J. Biol. Chem. 272: 23398-23406, 1997. PubMed: 9287355 Ray P, et al. Selective up-regulation of cytokine-induced RANTES gene expression in lung epithelial cells by overexpression of IkappaBR. J. Biol. Chem. 272: 20191-20197, 1997. PubMed: 9242696 Shimomura T, et al. Hepatocyte growth factor activator inhibitor, a novel Kunitz-type serine protease inhibitor. J. Biol. Chem. 272: 6370-6376, 1997. PubMed: 9045658 Kumar AS, et al. Phorbol ester down-regulation of lung surfactant protein B gene expression by cytoplasmic trapping of thyroid transcription factor-1 and hepatocyte nuclear factor 3. J. Biol. Chem. 272: 20764-20773, 1997. PubMed: 9252399 Guo FH, et al. Interferon gamma and interleukin 4 stimulate prolonged expression of inducible nitric oxide synthase in human airway epithelium through synthesis of soluble mediators. J. Clin. Invest. 100: 829-838, 1997. PubMed: 9259582 |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
