產品名稱 |
pHV33 |
商品貨號 |
B211492 |
Designations |
pHV33 |
Depositors |
Miles Laboratories, Inc., KL Wollweber, Miles Laboratories, Inc. |
U.S. Patent |
|
Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
Biosafety Level |
1 |
Host |
Distribution host: Escherichia coli RR1; K-12 RR1 |
Vector Information |
Size (kb): 6.9000000953674320 Vector: pHV33 (plasmid) Marker(s):ampR,cmlR,tetR Construct size (kb): 6.900000095367432 Features: marker(s): ampR, cmlR, tetR replicon: pMB1 |
Applications |
shuttle vector |
Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--7.9; HindIII--4.65, 3.05. This is an unstable, tetracycline-resistant mutant of pHV14. |
Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
Growth Conditions |
Temperature: 37.0°C |
References |
Primrose SB, Ehrlich SD. Isolation of plamid deletion mutants and study of their instability. Plasmid 6: 193-201, 1981. PubMed: 7029580
|
Shipped |
freeze-dried |
Shipping Information |
Distributed: freeze-dried |