亚洲精品无码成人片久久不卡,亚洲自偷自偷在线传媒,亚洲精品在线,久久无码精品九号,欧洲av人人爽爽,亚洲精品蜜桃久久久久久,亚洲精品久久午夜无码专区电影,辽宁少妇高潮45分钟,少妇高清性色生活片,无码八少妇久久

Distribution host: Escherichia coli JM109

Size (kb): 7.0000000000000000

DESCRIPTION OF VECTOR:
Intact vector size: 7.000
Type of vector: plasmid
Cloning sites: PstI HincII PvuI ScaI SmaI
Polylinker sites:
Construction: pDM2, pBGS8
Host range: Haemophilus influenzae; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, cmLR
replicon: pMB1, pRSF0885

Vector: pVT66 (plasmid)

Construction: pDM2, pBGS8

Marker(s):ampR,cmlR

Construct size (kb): 7.0

Features: marker(s): ampR, cmlR
replicon: pMB1, pRSF0885

shuttle vector

Restriction digests of the clone give the following sizes (kb): PstI--7.0; PvuI--7.0; SmaI--7.0; BamHI--4.7, 2.4.

Cloning into the HincII, PstI, PvuI, or ScaI sites inactivates ampR.

Cloning into the SmaI site inactivates cmlR.

Precautions have not been taken to maintain the F' in the JM109 host. The F' is not required to use this plasmid.

Shuttle vector.

The order of the major features in this plasmid is: pMB1 ori - ampR - pRSF0885 rep - cmlR.

Temperature: 37.0°C