Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.2, 2.2; PvuII--3.0, 1.85, 0.55; HindIII--5.4; PstI--4.3, 0.65, 0.45; BamHI--5.4. A-19 contains the complete coding sequence but not the complete 5' or 3' non-coding regions. Contains the following base substitutions relative the the published sequence for the normal B(+) variant: C(33) to G, G(202) to A (Val to Met), and A(376) to G (Asn to Asp). |